The Rat GPX1 (Glutathione Peroxidase 1) ELISA Kit is designed for the accurate quantification of GPX1 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.GPX1 is an important antioxidant enzyme that plays a key role in protecting cells from oxidative damage by reducing hydrogen peroxide and lipid peroxides. Dysregulation of GPX1 has been implicated in various diseases such as cancer, inflammatory conditions, and neurodegenerative disorders, making it a valuable biomarker for investigating these pathologies and potential therapeutic interventions. By utilizing the Rat GPX1 ELISA Kit, researchers can gain valuable insights into the role of GPX1 in health and disease, leading to a better understanding of cellular oxidative stress and potential treatment strategies.
Product Name:
Rat GPX1 (Glutathione Peroxidase 1) ELISA Kit
SKU:
RTES01107
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
