The Rat IFN-γ (Interferon Gamma) ELISA Kit is a highly specialized assay designed for the quantitative detection of IFN-γ levels in rat biological samples. IFN-γ is a critical cytokine involved in various immune responses, particularly in activating macrophages, stimulating natural killer cells, and modulating antigen presentation. This ELISA kit offers exceptional sensitivity and specificity in detecting and measuring IFN-γ concentrations, enabling researchers to accurately quantify this vital cytokine in rat samples. IFN-γ plays a key role in both innate and adaptive immunity, making it an essential factor in studying immune responses, infections, and immune-related diseases. Manufactured to stringent quality control standards, this ELISA kit provides robust and reliable performance, delivering reproducible results for researchers investigating the role of IFN-γ in immune regulation, inflammation, and host defense mechanisms in rats.
Product Name:
Rat IFN-gamma (Interferon Gamma) ELISA Kit
SKU:
AEES00292
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
