The Rat Immunoglobulin A (IgA) ELISA Kit is a specialized assay meticulously designed for the precise and quantitative measurement of Immunoglobulin A levels in various rat biological samples. Immunoglobulin A is a key component of the immune system, particularly known for its pivotal role in mucosal immunity and defense against pathogens at mucosal surfaces such as the respiratory and gastrointestinal tracts. This ELISA kit allows researchers to accurately determine IgA levels, providing valuable insights into the immune response, mucosal defense mechanisms, and antibody-mediated protection. By offering exceptional sensitivity and specificity, this kit enables detailed analysis of IgA concentrations, crucial for understanding the body's immune defenses and potential alterations in immunological responses. The Rat IgA ELISA Kit by Assay Genie delivers reliable and reproducible results, facilitating in-depth investigations into the dynamic role of Immunoglobulin A in immune surveillance and mucosal immunity. Designed and manufactured to stringent quality control standards, this kit ensures robust and dependable performance while offering ease of use and precision, making it an excellent choice for immunological research and studies focusing on mucosal immune responses.
Product Name:
Rat IgA (Immunoglobulin A) ELISA Kit
SKU:
AEES00530
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
