The Rat IgM (Immunoglobulin M) ELISA Kit is a state-of-the-art assay designed to quantitatively measure the levels of IgM, a critical component of the immune system, in rat biological samples. IgM is the first antibody produced during the initial immune response to infection, playing a pivotal role in neutralizing pathogens and activating the immune system. Accurate measurement of IgM levels is essential for understanding immune function and immune responses in rats, providing valuable insights into infection, autoimmunity, and inflammatory conditions. This advanced ELISA kit enables researchers to precisely quantify IgM levels in rat samples, shedding light on the immune status and responses in experimental models. IgM has significant implications in immune defense mechanisms and disease pathogenesis, making the Rat IgM ELISA Kit a valuable tool for immunology research and preclinical studies. With exceptional sensitivity and specificity, the Rat IgM ELISA Kit from Assay Genie ensures precise and reproducible results. Manufactured under strict quality control standards, this kit delivers reliable performance and ease of use, making it an indispensable choice for researchers investigating the role of IgM in immune function and disease processes in rat models.
Product Name:
Rat IgM (Immunoglobulin M) ELISA Kit
SKU:
AEES00660
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
