The Rat IL-5 ELISA Kit is expertly designed for the precise and quantitative detection of Interleukin-5 (IL-5) levels in diverse rat biological samples. IL-5 is a crucial cytokine known for its involvement in promoting the differentiation, maturation, and activation of various immune cells, especially eosinophils. As a key regulator of the immune response, IL-5 plays a significant role in allergic responses, asthma, and other immune-mediated conditions. Accurate measurement of IL-5 levels is essential for unraveling its specific functions in immune modulation and inflammatory processes. Assay Genie's Rat IL-5 ELISA Kit offers unparalleled sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control standards, this kit delivers robust performance, making it an ideal tool for research into the roles of IL-5 in immune responses and inflammatory disorders. The kit's user-friendly design further enhances its utility, providing researchers with a reliable and efficient solution for cytokine quantification studies.
Product Name:
Rat IL-5 ELISA Kit
SKU:
AEES00452
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
