The Rat INS (Insulin) ELISA Kit is a specialized assay designed for the accurate and precise quantification of insulin levels in various biological samples collected from rat models. Insulin is a critical hormone involved in glucose metabolism, cell growth, and energy storage processes, playing a pivotal role in regulating blood sugar levels and overall metabolic homeostasis. This ELISA kit provides researchers with a reliable tool to quantify insulin concentrations, allowing for the investigation of insulin secretion, metabolism, and its impact on various physiological and pathological conditions. Accurate measurement of insulin is essential for understanding its role in diabetes, metabolic disorders, and other related conditions. Manufactured under strict quality control standards, the Rat INS ELISA Kit ensures exceptional sensitivity and specificity, enabling researchers to obtain reliable and reproducible results. Its robust performance and ease of use make it an excellent choice for studies focused on insulin biology and metabolic research in rat models.
Product Name:
Rat INS (Insulin) ELISA Kit
SKU:
AEES00548
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
3.75 pg/mL
Detection range:
6.25-400 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
