The Rat LEP (Leptin) ELISA Kit is a highly sensitive assay meticulously designed for the quantitative assessment of Leptin levels in various rat biological samples. Leptin is a pivotal hormone that regulates energy balance, satiety, and body weight by signaling the brain to reduce appetite and increase energy expenditure. Imbalances in Leptin levels have been associated with obesity, metabolic disorders, and other health conditions, making its accurate measurement essential for research into metabolic mechanisms and potential therapeutic strategies. With exceptional sensitivity and specificity, our ELISA kit ensures precise and reproducible results, enabling researchers to explore the intricate role of Leptin in metabolic homeostasis effectively. Manufactured under stringent quality standards, this kit from Assay Genie provides reliable performance and user-friendly protocols, making it an ideal choice for investigative studies in the field of metabolism and energy regulation in rat models.
Product Name:
Rat LEP (Leptin) ELISA Kit
SKU:
AEES00466
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
