The Rat Luteinizing Hormone (LH) ELISA Kit is specifically designed for the quantitative measurement of LH levels in various rat biological samples. LH is a crucial hormone that plays a pivotal role in the regulation of reproductive processes, including ovulation and steroidogenesis. As a key gonadotropin, LH is essential for the maturation of ovarian follicles and the production of sex hormones, making it an important marker in reproductive endocrinology studies in rats. This ELISA kit from Assay Genie provides researchers with a reliable and sensitive tool for assessing LH levels, enabling detailed investigations into the reproductive hormone dynamics and fertility parameters in rat models. The kit ensures high specificity and precision, allowing for accurate and reproducible results. Manufactured under strict quality control measures, this ELISA kit offers robust performance and user-friendly protocols, making it an excellent choice for researchers in the field of reproductive biology, endocrinology, and fertility studies.
Product Name:
Rat LH (Luteinizing Hormone) ELISA Kit
SKU:
AEES00301
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 mIU/mL
Detection range:
1.56-100 mIU/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
