Rat MyD88 (myeloid differentiation factor 88) ELISA Kit
The Rat MyD88 (Myeloid Differentiation Factor 88) ELISA Kit is a powerful tool for the precise measurement of MyD88 levels in rat serum, plasma, and tissue culture supernatants. This advanced kit boasts exceptional sensitivity and specificity, ensuring consistent and dependable results for a variety of research purposes. MyD88 is a key signaling protein that plays a vital role in the immune response, particularly in the activation of inflammatory pathways and the defense against infections. Dysregulation of MyD88 has been implicated in various inflammatory disorders, autoimmune diseases, and cancer, making it a critical target for investigation and potential therapeutic interventions.
This state-of-the-art ELISA kit provides researchers with a valuable tool for studying MyD88 and its involvement in various pathological processes, offering insights that could lead to the development of novel treatment strategies. Trust in the Rat MyD88 ELISA Kit for precise and reliable detection of MyD88 levels in rat samples, advancing your research in immunology, inflammation, and beyond.
Product Name:
Rat MyD88 (myeloid differentiation factor 88) ELISA Kit
SKU:
RTFI01303
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
46.875 pg/mL
Range:
78.125-5000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
89-100%
84-99%
81-99%
EDTA Plasma (n = 5)
86-105%
83-97%
81-95%
Heparin Plasma (n = 5)
86-102%
82-98%
82-93%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
92-104
97
EDTA Plasma (n = 5)
90-97
93
Heparin Plasma (n = 5)
90-103
97
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Biotin-labeled Antibody (Concentrated, 100X)
60 ul
120 ul
2-8°C (Avoid direct light)
HRP-Streptavidin Conjugate (SABC, 100X)
60 ul
120 ul
2-8°C (Avoid direct light)
TMB Substrate
5 ml
10 ml
2-8°C (Avoid direct light)
Sample Dilution Buffer
10 ml
20 ml
2-8°C
Antibody Dilution Buffer
5 ml
10 ml
2-8°C
SABC Dilution Buffer
5 ml
10 ml
2-8°C
Stop Solution
5 ml
10 ml
2-8°C
Wash Buffer(25X)
15 ml
30 ml
2-8°C
Plate Sealer
3 pieces
5 pieces
-
Technical Manual
1 copy
1 copy
-
Abdel-Aziz et al.
TLR4/ MyD88/NF-κB signaling pathway involved in the protective effect of diacerein against lung fibrosis in rats
Rivaroxaban Modulates TLR4/Myd88/NF-Kβ Signaling Pathway in a Dose-Dependent Manner With Suppression of Oxidative Stress and Inflammation in an Experimental Model of Depression
