The Rat NGF (Nerve Growth Factor) ELISA Kit is a specialized assay designed for the quantitative detection of NGF levels in various rat biological samples. NGF is a critical neurotrophic factor known for its role in promoting the growth, survival, and differentiation of neurons, making it essential for the development and maintenance of the nervous system. Abnormal NGF levels have been implicated in various neurological conditions, including neurodegenerative diseases, neural injuries, and pain disorders, highlighting the significance of accurate NGF measurement in research and clinical studies. Accurate quantification of NGF through this ELISA kit enables researchers to investigate the neurotrophic effects of NGF, study neuronal pathophysiology, and explore potential therapeutic interventions targeting NGF signaling pathways. By measuring NGF levels in rat samples, researchers can gain insights into the role of this growth factor in neural health and disease progression. The Rat NGF ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under rigorous quality control standards, this kit delivers reliable performance and ease of use, making it an excellent choice for neuroscience research and drug discovery studies focusing on NGF biology.
Product Name:
Rat NGF (Nerve Growth Factor) ELISA Kit
SKU:
AEES00492
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
23.44 pg/mL
Detection range:
39.06-2500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
