The Rat OPN (Osteopontin) ELISA Kit is meticulously tailored for the precise quantification of Osteopontin levels in a variety of rat biological samples. Osteopontin, a multifunctional cytokine and extracellular matrix protein, is renowned for its diverse roles in cell adhesion, migration, inflammation, tissue remodeling, and immune responses. As a key modulator of physiological and pathological processes, Osteopontin is implicated in numerous conditions encompassing cancer, cardiovascular diseases, and bone metabolism. Accurate measurement of Osteopontin utilizing this ELISA kit offers researchers invaluable insights into the regulatory functions of Osteopontin in health and disease. Understanding the intricate involvement of Osteopontin in cellular signaling pathways, immune modulation, and disease progression is pivotal for discerning potential therapeutic avenues and diagnostic applications in various medical fields. The Rat OPN (Osteopontin) ELISA Kit from Assay Genie guarantees exceptional sensitivity and specificity, ensuring precise and reproducible outcomes. With a focus on stringent quality control measures, this kit ensures robust performance and user-friendly operation, making it an optimal selection for cutting-edge research endeavors exploring the multifaceted roles of Osteopontin in biological systems.
Product Name:
Rat OPN (Osteopontin) ELISA Kit
SKU:
AEES00501
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
