Rat PCSK9 (Proprotein convertase subtilisin/kexin type 9) ELISA kit
The Rat PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) ELISA Kit is specifically designed for the accurate measurement of PCSK9 levels in rat serum, plasma, and cell culture supernatants. With a high level of sensitivity and specificity, this kit provides reliable and reproducible results, making it an ideal tool for researchers in various fields.PCSK9 is a vital protein involved in the regulation of cholesterol levels in the body. Dysregulation of PCSK9 has been linked to cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions. By using the Rat PCSK9 ELISA Kit, researchers can gain valuable insights into the role of PCSK9 in disease progression and treatment strategies.
Product Name:
Rat PCSK9 (Proprotein convertase subtilisin/kexin type 9) ELISA kit
SKU:
RTES01104
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
