The Rat Prolactin (PRL) ELISA Kit offers a cutting-edge solution for the quantitative measurement of Prolactin levels in diverse rat biological samples. Prolactin, a pivotal hormone with multifaceted roles in reproduction, parental behavior, and metabolic regulation, plays a critical function in the endocrine system. By accurately quantifying Prolactin concentrations, researchers gain valuable insights into its physiological activities and its involvement in various biological processes. Accurate assessment of Prolactin levels is crucial for elucidating its impact on reproductive health, lactation, and metabolic functions in rat models. The Rat PRL ELISA Kit provides researchers with a reliable tool to explore the complex interplay of Prolactin in different physiological pathways, paving the way for a deeper understanding of its regulatory mechanisms and potential therapeutic targets. Engineered with precision and reliability in mind, this ELISA kit ensures exceptional sensitivity and specificity, guaranteeing consistent and reproducible results. Researchers can trust in the robust performance of the Rat PRL ELISA Kit to facilitate their research endeavors and advance scientific discoveries in the field of endocrinology and hormonal regulation.
Product Name:
Rat PRL (Prolactin) ELISA Kit
SKU:
AEES00522
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
