The Rat SMAD4/MADH4 ELISA Kit is a highly sensitive and reliable assay designed for the accurate detection of SMAD4 levels in rat serum, plasma, and cell culture supernatants. This kit is ideal for researchers studying the function of SMAD4 in various biological processes, including cell growth, differentiation, and apoptosis. SMAD4, also known as MADH4, is a critical protein in the TGF-β signaling pathway, regulating gene expression and cellular responses to growth factors.
Dysregulation of SMAD4 has been linked to numerous diseases, including cancer, fibrosis, and autoimmune disorders, making it a key target for therapeutic development. With its innovative design and proven performance, the Rat SMAD4/MADH4 ELISA Kit provides researchers with a powerful tool for investigating the role of SMAD4 in health and disease. Trust Assay Genie for accurate and reproducible results in your research studies.
Product Name:
Rat Smad4/MADH4 ELISA Kit
SKU:
RTFI01134
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
18.75 pg/mL
Range:
31.25-2000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
86-102%
85-103%
86-103%
EDTA Plasma (n = 5)
87-95%
90-96%
84-101%
Heparin Plasma (n = 5)
82-96%
85-93%
82-99%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
86-105
95
EDTA Plasma (n = 5)
92-97
94
Heparin Plasma (n = 5)
92-105
98
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
