Rat A Disintegrin And Metalloprotease 17 (ADAM17) ELISA Kit
The Rat ADAM17 (A Disintegrin and Metalloprotease 17) ELISA Kit is designed for the accurate detection of ADAM17 levels in rat serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results, making it ideal for a wide range of research applications.ADAM17 is a key enzyme involved in the shedding of cell surface proteins, playing a critical role in various biological processes such as inflammation, cell proliferation, and tumorigenesis.
Dysregulation of ADAM17 has been implicated in various diseases including cancer, Alzheimer's, and inflammatory disorders, making it a valuable biomarker for research and potential therapeutic development.With easy-to-follow protocols and all necessary components included, the Rat ADAM17 ELISA Kit offers a convenient and efficient solution for studying ADAM17 expression and activity in rat samples. Trust Assay Genie for accurate and reliable results in your research endeavors.
Product Name:
Rat A Disintegrin And Metalloprotease 17 (ADAM17) ELISA Kit
Product Code:
RTDL00018
Size:
96 Assays
Target:
ADAM17
Synonyms:
CD156b, TACE, CSVP, Tumor Necrosis Factor,Alpha,Converting Enzyme, Disintegrin and metalloproteinase domain-containing protein 17, Snake venom-like protease
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
