The Rat TAT (Thrombin-Antithrombin) Complex ELISA Kit is specifically designed for the precise quantification of thrombin-antithrombin complex levels in rat samples, including serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.The thrombin-antithrombin complex is a key indicator of the coagulation cascade, reflecting the balance between thrombin production and antithrombin inhibition. Dysregulation of this complex is associated with various clotting disorders, thrombotic events, and inflammatory processes. By accurately measuring TAT levels, researchers can gain valuable insights into coagulation dynamics and identify potential therapeutic targets for conditions such as disseminated intravascular coagulation and sepsis.Overall, the Rat TAT Complex ELISA Kit is a valuable tool for studying coagulation disorders and exploring novel interventions to modulate thrombin activity and antithrombin function in preclinical rat models.
Product Name:
Rat TAT (Thrombin-Antithrombin Complex) ELISA Kit
SKU:
RTES00799
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
