ACVR2A and ACVR2B are two activin type II receptors. ACVR2B is integral to the activin and myostatin signaling pathway. Ligands such as activin and myostatin bind to ACVR2A and ACVR2B. Myostatin, a negative regulator of skeletal muscle growth, is regarded as a potential therapeutic target and binds to ACVR2B effectively, and to a lesser extent, to ACVR2A. The structure of human ACVR2B kinase domain in complex with adenine establishes the conserved bilobal architecture consistent with all other catalytic kinase domains. Haplotype structure at the ACVR2B and follistatin loci may contribute to interindividual variation in skeletal muscle mass and strength. Defects in ACVR2B are a cause of left-right axis malformations.
Human ACVR2B/ActivinR-IIB Recombinant Protein (RPES5197)
Activin Receptor Type-2B, Activin Receptor Type IIB, ACTR-IIB, ACVR2B,Bone Morphogenetic Protein Receptor Type-2, BMP Type-2 Receptor, BMPR-5, Bone Morphogenetic Protein Receptor Type II, BMP Type II Receptor
Met 1-Thr 134
1. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.02-0.1 µg/mL in the presence of 10 ng/mL recombinant Activin A.2. Measured by its binding ability in a functional ELISA. 3. Immobilized human ACVR2B at 10 µg/mL (100 ?l/well) can bind biotinylated human INHBA-His, The EC50 of biotinylated human INHBA-His is 0.112 µg/mL.4. Immobilized human ACVR2B at 10 µg/mL (100 ?l/well) can bind biotinylated mouse INHBA-His, The EC50 of biotinylated mouse INHBA-His is 0.161 µg/mL.
<1.0 EU per µg as determined by the LAL method.
A DNA sequence encoding the N-terminal segment (Met 1-Thr 134) from the extracellular domain of human ACVR2B (NP_001097.2) was expressed with the fused human IgG1 Fc region at the C-terminus.
> 97 % as determined by reducing SDS-PAGE.
AP Mol Mass:
Lyophilized from sterile PBS, pH 7.4
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.