ALPI encodes for intestinal phosphatase alkaline; a brush border metalloenzyme that hydrolyses phosphate from the lipid A moiety of lipopolysaccharides and thereby drastically reduces Toll-like receptor 4 agonist activity. ALPI mutations impaired either stability or catalytic activity of ALPI and rendered it unable to detoxify lipopolysaccharide-dependent signalling. ALPI mutations should be included in screening for monogenic causes of inflammatory bowel diseases and lay the groundwork for ALPI-based treatments in intestinal inflammatory disorders.
Alkaline Phosphatase/ALPI Recombinant Protein (RPES4373)
Met 1-Asp 503
Measured by its ability to cleave a fluorogenic substrate; 4-Methylumbelliferyl phosphate (4-MUP).The specific activity is > 1;000 pmoles/min/µg.
<1.0 EU per µg of the protein as determined by the LAL method.
A DNA sequence encoding the mature form of human ALPI (P09923) (Met 1-Asp 503);without the pro peptide; was fused with a polyhistidine tag at the C-terminus.
> 95 % as determined by reducing SDS-PAGE.
AP Mol Mass:
Lyophilized from sterile PBS; pH 7.4
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.