Description
system_update_altDatasheet
| Product Name: | AMPK (G1/B1/A2) Recombinant Protein (RPES0595) |
| Product Code: | RPES0595 |
| Size: | 50 µg |
| Species: | Human |
| Expressed Host: | Baculovirus-Insect Cells |
| Synonyms: | AMPK,AMPKa1 |
| Accession: | P54619 |
| Sequence: | Met 1-Pro 331 |
| Fusion tag: | |
| Activity: | The specific activity was determined to be 4 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate. |
| Endotoxin: | <1.0 EU per µg of the protein as determined by the LAL method. |
| Protein Construction: | A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331); constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (Q9Y478) (Met 1-Ile 270); constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus; constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified. |
| Purity: | > 94 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 158 (38+30+90) kDa |
| AP Mol Mass: | 40; 42 & 95 kDa |
| Formulation: | Supplied as sterile 50mM Tris; 200mM NaCl; 1mM EDTA; 1mM DTT; 0.5mM PMSF; 10% gly; 1mM GSH; pH 7.4 |
| Shipping: | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C. |
| Stability and Storage: | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
