BACE1 (beta-site APP-cleaving enzyme 1) is an aspartic-acid protease that is important in the formation of myelin sheaths in peripheral nerve cells. In the brain, it's found in significant quantities in the substantia nigra, locus coruleus, and medulla oblongata. In addition, strong BACE1 expression has been observed in pancreatic cells. BACE1 is involved in the development of Alzheimer's disease. BACE1 levels were increased in Alzheimer's disease patients, despite no changes in mRNA expression. BACE1 gene expression is controlled by a TATA-less promoter. Its expression is controlled via translational repression. The extremely low amounts of Ca(2+) (microM range) boosted the proteolytic activity of BACE1 considerably. Furthermore, BACE1 protein is ubiquitinated and the ubiquitin-proteasome pathway controls the degradation of BACE1 proteins and amyloid precursor protein. It has also been identified as the rate-limiting enzyme for amyloid-beta-peptide (Abeta) production. Human BACE1/ASP2 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human BACE1/ASP2 Recombinant Protein (RPES0508)
Product Code:
RPES0508
Size:
100 µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
ASP2,BACE,HSPC104
Accession:
NP_036236.1
Sequence:
Met 1-Thr 457
Fusion tag:
Activity:
Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dpn)-Arg-Arg-NH2 (Catalog# ES004; R&D Systems). Cleavage of ES004 can be measured using excitation and emission wavelength of 320 and 405 nm; respectively. The specific activity is >3.5 pmoles/min/µg.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
The mature form of human BACE1 (NP_036236.1) extracellular domain (Met1-Thr 457) with a quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
49 kDa
AP Mol Mass:
65 kDa
Formulation:
Lyophilized from sterile 50mM Tris; 100mM NaCl; pH 8.0
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
