Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Carbonic Anhydrase 9/CA9 Recombinant Protein (RPES2015)
CAIX,Carbonic Anhydrase IX,MN
Met 1-Asp 414
Measured by its esterase activity. The specific activity is >30 pmoles/min/µg, as measured with 1 mM 4-Nitrophenyl acetate and 2.5 µg enzyme at 400 nm in 100 ?L of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
<1.0 EU per µg as determined by the LAL method.
A DNA sequence encoding the human carbonic anhydrase IX (CA9) precursor (NP_001207.2) (Met 1-Asp 414) was expressed with a polyhistidine tag at the C-terminus.
> 95 % as determined by reducing SDS-PAGE.
AP Mol Mass:
Lyophilized from sterile PBS, pH 7.4
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.