Recombinant Human Carboxypeptidase A1/CPA1 Protein (His Tag) (RPES4399)

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SKU:
RPES4399
€361

Description

ELISA Kit Technical Manual

Recombinant Human Carboxypeptidase A1/CPA1 Protein (His Tag)

Background:Carboxypeptidase A1 (CPA1) is secreted as a pancreatic peptidase that comes from the precursor form of inactive procarboxypeptidase. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. It has a free C-terminal carboxyl group, with the preference of residues with aromatic or branched aliphatic side chains. CPA1 cleaves the C-terminal amide or ester bond of peptides and involves in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E.
Name:Recombinant Human Carboxypeptidase A1/CPA1 Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES4399
Synonyms:Carboxypeptidase A1; CPA1; CPA
Species:Human
Expression host:Human Cells
Sequence:Lys17-Tyr419
Accession:P15085
Mol Mass:46.6 kDa
AP Mol Mass:44 kDa
Tag:C-6His
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Formulation:Supplied as a 0.2 um filtered solution of 20mM TrisHCl, 150mm NaCl, pH 7.5.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:CPA1: Carboxypeptidase that catalyzes the release of a C- terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro. Belongs to the peptidase M14 family.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Protease; EC 3.4.17.1; Secreted

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: extracellular space

Molecular Function:zinc ion binding; metallocarboxypeptidase activity

Biological Process: proteolysis

NCBI Summary:Three different forms of human pancreatic procarboxypeptidase A have been isolated. This gene encodes a monomeric pancreatic exopeptidase involved in zymogen inhibition. [provided by RefSeq, Jan 2009]
UniProt Code:P15085
NCBI GenInfo Identifier:399196
NCBI Gene ID:1357
NCBI Accession:P15085.2
UniProt Secondary Accession:P15085,Q53XU0, Q9BS67, Q9UCF2, A4D1M1,
UniProt Related Accession:P15085
Molecular Weight:419
NCBI Full Name:Carboxypeptidase A1
NCBI Synonym Full Names:carboxypeptidase A1 (pancreatic)
NCBI Official Symbol:CPA1  
NCBI Official Synonym Symbols:CPA  
NCBI Protein Information:carboxypeptidase A1; pancreatic carboxypeptidase A
UniProt Protein Name:Carboxypeptidase A1
Protein Family:Carboxypeptidase
UniProt Gene Name:CPA1  
UniProt Entry Name:CBPA1_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Human
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