The Recombinant Human Estrogen receptor/ESR1 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Estrogen receptor/ESR1 function and its implications in health and disease.
This product (SKU: RPCB0786) is produced using E. coli and features a N-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 14.38 kDa with an observed molecular weight of 14 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human Estrogen receptor/ESR1 Protein
SKU:
RPCB0786
Size:
10 μg , 50 μg
Reactivity:
Human
Synonyms:
ER, ESR, ESRA, ESTRR, Era, NR3A1, ESR1, ESRα, ESR, ESRA, ESTRR, Era, NR3A1, ER alpha
High quality, high purity and low endotoxin recombinant Recombinant Human Estrogen receptor/ESR1 Protein (RPCB0786), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2Contact us for customized product form or formulation.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Estrogen Receptor is a major ligand--‐activated transcription factor belonging to the nuclear hormone receptor superfamily. Estrogen Receptor is composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but they also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.
Recombinant Human Estrogen receptor/ESR1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
