Recombinant Human TNFSF5/CD40 ligand/CD154 Protein
The Recombinant Human TNFSF5/CD40 ligand/CD154 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating TNFSF5/CD40 ligand/CD154 function and its implications in health and disease.
This product (SKU: RPCB1236) is produced using HEK293 cells and features a N-6His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 16.64 kDa with an observed molecular weight of 20-27 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human TNFSF5/CD40 ligand/CD154 Protein
High quality, high purity and low endotoxin recombinant Recombinant Human TNFSF5/CD40 ligand/CD154 Protein (RPCB1236), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Measured by its binding ability in a functional ELISA.Immobilized Human CD40L (RPCB1236) at 5 μg/mL (100 μL/well) can bind Human CD40 (RPCB0821) with a linear range of 0.005-0.5 μg/mL.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen-presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-γ from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation.
Recombinant Human TNFSF5/CD40 ligand/CD154 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Human CD40L (RPCB1236) at 5 μg/mL (100 μL/well) can bind Human CD40 (RPCB0821) with a linear range of 0.005-0.5 μg/mL.
