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Human VNN1/Vanin Recombinant Protein (RPES2422)

SKU:
RPES2422
Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Human
€142

Description

ELISA Kit Technical Manual

Recombinant Human VNN1/Vanin Protein (His Tag)

Background:Vanin is a cell membrane protein which contains one CN hydrolase domain and belongs to the CN hydrolase family and BTD/VNN subfamily. Vanin is also a member of the Vanin family of proteins, which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. Vanin is widely expressed with higher expression in spleen, kidney and blood and overexpressed in lesional psoriatic skin. No biotinidase activity has been demonstrated for any of the vanin proteins; however, they possess pantetheinase activity, which may play a role in oxidative-stress response. Vanin is an epithelial pantetheinase that provides cysteamine to tissue and regulates response to stress. Vanin is expressed by enterocytes, and its absence limits intestinal epithelial cell production of proinflammatory signals. Vanin regulates late adhesion steps of thymus homing under physiological, noninflammatory conditions. The early impact of vanin deficiency on tumor induction was directly correlated to the amount of inflammation and subsequent epithelial proliferation rather than cell death rate. Vanin molecule was shown to be involved in the control of thymus reconstitution following sub-lethal irradiation.
Name:Recombinant Human VNN1/Vanin Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES2422
Synonyms:Pantetheinase; Pantetheine Hydrolase; Tiff66; Vascular Non-Inflammatory Molecule 1; Vanin; VNN1
Species:Human
Expression host:Human Cells
Sequence:Gln22-Ser490
Accession:O95497
Mol Mass:53.3 kDa
AP Mol Mass:83 kDa
Tag:C-6His
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:VNN1: Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. Belongs to the CN hydrolase family. BTD/VNN subfamily.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; EC 3.5.1.92; Hydrolase; Membrane protein, integral; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Cell adhesion; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 6q23-q24

Cellular Component: integral to membrane; plasma membrane

Molecular Function:pantetheine hydrolase activity

Biological Process: positive regulation of T cell differentiation in the thymus; chronic inflammatory response; cell-cell adhesion; pantothenate metabolic process; innate immune response; response to oxidative stress; cell motility; inflammatory response; acute inflammatory response

NCBI Summary:This gene encodes a member of the vanin family of proteins, which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. This protein, like its mouse homolog, is likely a GPI-anchored cell surface molecule. The mouse protein is expressed by the perivascular thymic stromal cells and regulates migration of T-cell progenitors to the thymus. This gene lies in close proximity to, and in the same transcriptional orientation as, two other vanin genes on chromosome 6q23-q24. [provided by RefSeq, Feb 2009]
UniProt Code:O95497
NCBI GenInfo Identifier:76803830
NCBI Gene ID:8876
NCBI Accession:O95497.2
UniProt Related Accession:O95497
Molecular Weight:
NCBI Full Name:Pantetheinase
NCBI Synonym Full Names:vanin 1
NCBI Official Symbol:VNN1  
NCBI Official Synonym Symbols:HDLCQ8; Tiff66  
NCBI Protein Information:pantetheinase
UniProt Protein Name:Pantetheinase
UniProt Synonym Protein Names:Pantetheine hydrolase; Tiff66; Vascular non-inflammatory molecule 1; Vanin
Protein Family:Pantetheinase
UniProt Gene Name:VNN1  
UniProt Entry Name:VNN1_HUMAN