RCN3 belongs to the CREC family which contains multiple EF-hand Ca2+-binding proteins localized to the secretory pathway. RCN3 sequence is characterized by the presence of five Arg-Xaa-Xaa-Arg motifs, which represents the target sequence of subtilisin-like proprotein convertases(SPCs). SPCs are a family of seven structurally related serine endoproteases that are involved in the proteolytic activation of proproteins.RCN3 is transiently associated with proPACE4, but not with mature PACE4. Inhibition of PACE4 maturation by a Ca2+ ionophore resulted in accumulation of the proPACE4-RCN-3 complex in cells. It has been proposed that elective and transient association of RCN3 with the precursor of PACE4 plays an important role in the biosynthesis of PACE4.
Product Name:
Mouse Reticulocalbin 3/RCN3 Recombinant Protein (RPES2077)
Product Code:
RPES2077
Size:
20µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
6030455P07Rik,D530026G20Rik,D7Ertd671e,RLP49
Accession:
NP_080831.2
Sequence:
Met1-Leu328
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse Rcn3 (NP_080831.2) (Met1-Leu328) was expressed with a polyhistidine tag at the C-terminus.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
36.9 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
