IFN gamma, also known as IFNG, is a secreted protein which belongs to the type I I interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Rat Interferon gamma/IFNG Recombinant Protein (RPES3869)
Val 117-Ser 268
1. Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 1-5 ng/mL.2. Measured by its binding ability in a functional ELISA. Immobilized rat IFNG-Fc at 10 µg/ml (100 ?l/well) can bind biotinylated rat IFNGR-Fc. The EC50 of biotinylated rat IFNGR-Fc is 20.1-46.9 ng/ml.
<1.0 EU per µg of the protein as determined by the LAL method
A DNA sequence encoding the rat IFNG (P01581) (Met 1-Cys 156) was fused with the Fc region of human IgG1 at the C-terminus.
> 92 % as determined by SDS-PAGE
AP Mol Mass:
Lyophilized from sterile PBS, pH 7.4
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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