The SARS-CoV-2 Neutralization Antibody ELISA Kit (semi-quantitative) is designed for the quantitative detection of Human SARS-CoV-2 Neutralization Antibody levels in various biological samples. SARS-CoV-2, the causative agent of COVID-19, is a betacoronavirus that primarily infects cells of the respiratory tract via binding of its Spike (S) protein to the angiotensin-converting enzyme 2 (ACE2) receptor. Humoral immune responses to SARS-CoV-2 infection and vaccination are characterized by the generation of antibodies targeting the Spike protein, receptor-binding domain (RBD), and nucleocapsid protein. Quantitative measurement of SARS-CoV-2-specific antibodies, including neutralizing antibodies that block viral entry, is essential for seroepidemiological studies, vaccine immunogenicity assessment, convalescent plasma screening, and monitoring of immune protection duration. Assay Genie's SARS-CoV-2 Neutralization Antibody ELISA Kit (semi-quantitative) offers exceptional sensitivity and specificity with a sensitivity of 9.38 ng/mL, ensuring reliable and reproducible results across a broad range of sample types including serum, plasma. Manufactured under stringent quality control standards, this ELISA kit provides robust performance and ease of use, making it an outstanding choice for both research and clinical applications. Trust Assay Genie's SARS-CoV-2 Neutralization Antibody ELISA Kit (semi-quantitative) for accurate and dependable quantification of this crucial biomarker in your studies.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
This kit recognizes Human SARS-CoV-2 Neutralization Antibody in samples.No significant cross-reactivity or interference between Human SARS-CoV-2 Neutralization Antibody and analogues was observed
