The T4 (Thyroxine) ELISA Kit is a highly specialized assay designed for the precise quantitative detection of Thyroxine (T4) levels in various biological samples. Thyroxine, an important thyroid hormone produced by the thyroid gland, plays a pivotal role in regulating metabolism, growth, and development in the body. It is a crucial component of the endocrine system and influences numerous physiological processes, including energy production, protein synthesis, and temperature regulation. By measuring T4 levels with this ELISA kit, researchers can gain valuable insights into thyroid function, metabolic activity, and hormonal balance within biological samples. Thyroxine serves as a key marker for thyroid health and can provide essential information on thyroid disorders, metabolic conditions, and other health-related concerns. The T4 ELISA Kit offers exceptional sensitivity and specificity, enabling accurate and reproducible results for researchers studying thyroid function and related physiological processes. Manufactured under strict quality control standards, this kit ensures robust performance and reliable data, making it an ideal choice for research studies focused on thyroid hormones, metabolism, and endocrine health.
Product Name:
T4 (Thyroxine) ELISA Kit
SKU:
AEES00694
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
