TCP1-alpha Antibody [91a] is a rat monoclonal antibody directed against TCP1-alpha, offered by Assay Genie for research applications. Reported applications include WB, ICC/IF, IP and FCM. Reported reactivity: Human, Mouse, Rat, Bovine, Rabbit, Guinea Pig (Cavia porcellus), Hamster, Dog, Pig, Yeast, Yeast (Saccharomyces cerevisiae), Nematode (Caenorhabditis elegans), Fruit Fly (Drosophila melanogaster) and Monkey. Supplied as IgG2a, purified by Protein G.
T-complex protein 1 subunit alpha (TCP1α) is a core component of the eukaryotic chaperonin-containing TCP1 complex (CCT or TRiC), a cytosolic molecular chaperone essential for the ATP-dependent folding of key cytoskeletal proteins such as actin and tubulin. TCP1α is ubiquitously expressed and highly conserved, playing a central role in maintaining proteostasis under both physiological and stress conditions. As part of the CCT complex, TCP1α assists in the proper folding of a wide range of client proteins involved in cell structure, intracellular transport, and signal transduction. Like heat shock proteins (e.g., HSP70), TCP1α is upregulated in response to cellular stress and exhibits ATPase activity, underscoring its role in protein quality control. In the context of neurodegenerative disease, TCP1α has gained attention for its involvement in mitigating protein misfolding and aggregation—hallmarks of disorders such as Alzheimer’s, Parkinson’s, and Huntington’s disease. Impaired chaperonin function can lead to the accumulation of toxic protein species, disrupted cytoskeletal dynamics, and neuronal dysfunction. Moreover, TCP1α may influence the degradation of misfolded proteins via the ubiquitin-proteasome system and autophagy pathways. Given its critical role in neuronal proteostasis and cytoskeletal integrity, TCP1α is emerging as a potential therapeutic target for modulating protein folding and preventing neurodegeneration. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.
Product Name:
TCP1-alpha Antibody [91a]
Product SKU:
STMA00164
Size:
100 µg
Target:
TCP1-alpha
Host Species:
Rat
Antibody Type:
Monoclonal
Clone ID:
91a
Research Areas:
Cancer, Heat Shock, Cell Signaling, Protein Trafficking
Buffer:
PBS pH7.4, 50% glycerol, 0.1% sodium azide
Storage & Shipping:
Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Specificity:
Detects ~60kDa. Also detects ~92kDa. Cross reactivity with human HSP60 has been observed with this antibody in immunoblot analysis. Reacts weakly with Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recognizes TCP1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome.
Guarantee:
12 months from date of dispatch
Immunogen:
Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region).
