Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit (BN00828)

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BN00828
  • Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit (BN00828)
  • Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit (BN00828)
  • Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit (BN00828)
€631

Description

ELISA Kit Technical ManualMSDS

Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit

The Cholesterol/Cholesteryl Ester Quantitation Kit provides a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both by colorimetric or fluorometric methods. Majority of the cholesterol in blood is in the form of cholesteryl esters which can be hydrolyzed to cholesterol by cholesterol esterase. Cholesterol is then oxidized by cholesterol oxidase to yield Hhich reacts with a sensitive cholesterol probe to produce color (ax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The assay detects total cholesterol (cholesterol and cholesteryl esters) in the presence of cholesterol esterase or free cholesterol in the absence of cholesterol esterase in the reaction. Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total (cholesterol plus cholesteryl esters).

Figure:  (a) Cholesterol Standard Curve, colorimetric (b) Cholesterol Standard Curve, fluorometric (c) Quantification of Cholesterol/Cholesterol Ester from normal human serum (1 µl) and serum from patient with atherosclerosis (1 µl). Assay was performed following the kit protocol.

Key information Description

Product SKU

BN00828

Size

100 assays

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Applications

The assay detects total cholesterol (cholesterol and cholesteryl esters) in the presence of cholesterol esterase or free cholesterol in the absence of cholesterol esterase in the reaction.

Features and Benefits

  • Simple procedure; takes ~60 minutes
  • Fast and convenient

Kit Components

  • Cholesterol Assay Buffer
  • Cholesterol Probe (in DMSO, anhydrous)
  • Enzyme Mix (lyophilized)
  • Cholesterol Esterase (lyophilized)
  • Cholesterol Standard (2 µg/µl)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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