Triglyceride Quantification Colorimetric Assay Kit (384-well) (BN01113)

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SKU:
BN01113
  • Triglyceride Quantification Colorimetric Assay Kit (384-well) (BN01113)
  • Triglyceride Quantification Colorimetric Assay Kit (384-well) (BN01113)
€714

Description

ELISA Kit Technical ManualMSDS

Triglyceride Quantification Colorimetric Assay Kit (384-well)

Triglycerides (TGs)are the main constituent of body fat in humans and animals, as well as vegetable fat. TGs are found as a natural component in human's blood circulatory system. TGs are composed of one molecule of glycerol and 3 molecules of fatty acids. The metabolism of cholesterol generates fatty acids and glycerol, and both can serve as substrates in metabolic pathways to produce energy. Excess TGs can remain in the body as fatty deposits and that may lead to obesity.Elevated triglyceride levels also increase the risk for heart disease, strokes, and type 2 diabetes. Assay Genie's Triglyceride Quantification Kit provides a sensitive, easy assay to measure TG concentration in a variety of samples. In the assay, TGs are converted to free fatty acids and glycerol. The released glycerol is then oxidized to generate a product which reacts with a probe to generate a color that can be detected by a spectrophotometer at = 590 nm. The method is quantitative, rapid, simple, sensitive, and designed for high throughput format.The kit provides a convenient means for detecting 0.5 to 5 mM Triglycerides in biological samples

Figure: (a) Triglyceride Standard Curve. (b) Determination of Triglyceride in pooled normal human serum. Undiluted serum sample (2 µl) was assayed directly (no prior treatment) according to the kit protocol. Calculated concentration of triglyceride: 0.65 ± 0.02 mM; (58 ± 1.8 mg/dl).

Key Information Description

Product SKU

BN01113

Size

400 assays

Detection Method

Absorbance (590 nm)

Species Reactivity

Mammalians

Applications

  • The kit can detect 2 pmol-10nmol (or 0.5-5 mM range) of triglyceride in various samples.
  • The kit also detects monoglycerides and diglycerides.

Features and Benefits

  • Simple procedure; takes ~ less than 40 minutes
  • Fast and convenient

Kit Components

  • Triglyceride Assay Buffer
  • Triglyceride Probe (in DMSO, anhydrous)
  • Lipase (lyophilized)
  • Triglyceride Enzyme Mix (lyophilized)
  • Triglyceride Standard (1 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack.

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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