The VB6 (Vitamin B6) ELISA Kit is specifically designed for the precise measurement of Vitamin B6 levels in various samples including serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.Vitamin B6, also known as pyridoxine, is a vital nutrient that plays a crucial role in numerous biological processes such as metabolism, neurotransmitter synthesis, and immune function. Deficiencies in Vitamin B6 have been linked to various health issues including anemia, cognitive decline, and skin problems, highlighting the importance of monitoring Vitamin B6 levels for overall health and well-being.Whether studying the effects of Vitamin B6 supplementation, investigating its role in certain diseases, or simply monitoring levels for general health assessments, the VB6 ELISA Kit provides researchers with a reliable and efficient tool for accurate Vitamin B6 detection.
Product Name:
VB6 (Vitamin B6) ELISA Kit
SKU:
UNES00005
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
