The VD3 (Vitamin D3) ELISA Kit is specifically designed for the precise measurement of Vitamin D3 levels in various biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it an invaluable tool for research purposes.Vitamin D3, also known as cholecalciferol, plays a crucial role in regulating calcium and phosphorus levels in the body, promoting bone health, immune function, and overall well-being. Deficiencies in Vitamin D3 have been linked to a variety of health conditions, including osteoporosis, autoimmune diseases, and cardiovascular disorders, highlighting the importance of monitoring Vitamin D3 levels. By utilizing the VD3 ELISA Kit, researchers can gain valuable insights into Vitamin D3 status, assess potential deficiencies, and explore the impact of Vitamin D3 on various physiological processes. This kit provides a reliable and efficient way to measure Vitamin D3 levels, enabling researchers to advance their understanding of Vitamin D3 biology and its implications for human health.
Product Name:
VD3 (Vitamin D3) ELISA Kit
SKU:
UNES00010
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
