Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit (BN00930)

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BN00930
  • Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit (BN00930)
  • Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit (BN00930)
¥60,037.25

Description

ELISA Kit Technical ManualMSDS

Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit

Xanthine oxidase (XO, EC 1.17.3.2 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. However, in various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice. Assay Genie has developed an easy and sensitive assay to determine XO in variety of samples. In the assay, XO oxidizes xanthine to hydrogen peroxide (H which reacts stoichiometrically with GenieRed Probe to generate color (at = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to XO content, the XO activity can be accurately measured. The kit detects 1-100 mU xanthine oxidase in 100 µl reaction volume.

Figure: Xanthine Oxidase Standard Curve obtained using kit protocol and sample kinetics

Key Information Description

Product SKU

BN00930

Size

100 assays

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Applications

The kit detects 1-100 mU xanthine oxidase in 100 µl reaction volume.

Features and Benefits

  • Simple procedure; takes ~ less than 40 minutes
  • Fast and convenient

Kit Components

  • XO Assay Buffer
  • GenieRed Probe
  • DMSO (anhydrous)
  • XO Enzyme Mix
  • XO Substrate Mix
  • XO Positive Control (~200mU)
  • HStandard (0.88 M)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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