Recombinant ZIKV (strain Zika SPH2015) Envelope protein (Domain III, (RPES7018)
Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF.Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.
Product Name:
Recombinant ZIKV (strain Zika SPH2015) Envelope protein (Domain III,
SKU:
RPES7018
Size:
100μg
Species:
ZIKV
Synonyms:
E protein, Zika virus, strain Zika SPH2015
Tag:
C-His
Sequence:
Val593-Lys699
Expression Host:
Baculovirus-Insect Cells
Accession:
ALU33341.1
Endotoxin:
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Calculated MW:
13 kDa
Bio-Activity:
Not validated for activity
Form:
Lyophilized powder
Source:
Baculovirus-Insect Cells-derived ZIKV ZIKV (strain Zika SPH2015) Envelope protein Val593-Lys699, with an C-terminal His
Formulation:
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Storage:
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 ℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
