The Alpha-tubulin (ubiquitous) chain Rabbit pAb (CABC024) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables double-stranded RNA binding activity and ubiquitin protein ligase binding activity. Predicted to be involved in microtubule cytoskeleton organization and mitotic cell cycle. Predicted to act upstream of or within cellular response to interleukin-4. Located in microtubule.
This antibody is validated for use in WB, IHC-P, IF/ICC applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Alpha-tubulin (ubiquitous) chain Rabbit pAb
SKU:
CABC024
Size:
100μL, 200μL, 50μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICC
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
IF/ICC
1:50 - 1:200
Synonyms:
K-ALPHA-1, Alpha-tubulin (ubiquitous) chain
Positive Sample:
HeLa, PC-12, A-549, U-251MG, Mouse brain
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
50kDa
Observed MW:
52kDa
Enables double-stranded RNA binding activity and ubiquitin protein ligase binding activity. Predicted to be involved in microtubule cytoskeleton organization and mitotic cell cycle. Predicted to act upstream of or within cellular response to interleukin-4. Located in microtubule.
Purification Method
Affinity purification
Gene ID
10376
RRID
AB_2768343
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Alpha-tubulin (ubiquitous) chain Rabbit pAb (CABC024) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Alpha-tubulin (ubiquitous) chain Rabbit pAb (CABC024) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of NIH/3T3 cells using Alpha-tubulin (ubiquitous) chain Rabbit pAb (CABC024) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using Alpha-tubulin (ubiquitous) chain Rabbit pAb (CABC024) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
