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Cystatin B Monoclonal Antibody (CAB20886)

SKU:
CAB20886
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Plant
Host Species:
Rabbit
Isotype:
IgG
  • Western blot analysis of extracts of various cell lines, using Cystatin B antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using Cystatin B antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
$215 - $563
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Description

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Product Name:Cystatin B Monoclonal Antibody
SKU:CAB20886
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-98 of human Cystatin B (NP_000091.1).
Sequence:MMCG APSA TQPA TAET QHIA DQVR SQLE EKEN KKFP VFKA VSFK SQVV AGTN YFIK VHVG DEDF VHLR VFQS LPHE NKPL TLSN YQTN KAKH DELT YF
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:PME; ULD; CST6; EPM1; STFB; CPI-B; EPM1A; Cystatin B
Positive Sample:U-87MG,HL-60 (Low expression control),Mouse liver,Mouse kidney
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus
Calculated MW:11kDa
Observed MW:13kDa

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.

Purification Method:Affinity purification
Gene ID:1476
Clone Number:ARC2833
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using Cystatin B antibody (CAB20886) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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