The HCoV-OC43 Spike S2 Antibody (CAB20616) is a high-quality antibody developed for reliable detection and analysis of target proteins. S1 attaches the virion to the cell membrane by interacting with sialic acid-containing cell receptors, initiating the infection.; [Spike protein S1]: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against HCoV-OC43 samples.
Product Name:
HCoV-OC43 Spike S2 Antibody
SKU:
CAB20616
Size:
100μL, 20μL
Reactivity:
HCoV-OC43
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
Human coronavirus (HCoV-OC43) Spike Protein (S1+S2 ECDHis Tag)
Calculated MW:
150kDa
Observed MW:
75kDa/145kDa
S1 attaches the virion to the cell membrane by interacting with sialic acid-containing cell receptors, initiating the infection.; [Spike protein S1]: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.
Purification Method
Affinity purification
Gene ID
39105218
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Human coronavirus (HCoV-OC43) Spike Protein (S1+S2 ECDHis Tag), using HCoV-OC43 Spike S2 Rabbit pAb (CAB20616) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
