Description
Anti-Human CD71 In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Human CD71 In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD71 protein, pivotal in cellular iron uptake and proliferation, making it ideal for research in cellular biology and related fields. With a rat IgG2a isotype, it ensures high purity and ultra-low endotoxin levels (<0.01 EU/µg), perfect for in vivo studies, ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy.
Enhance your research with this reliable and versatile antibody. CD71, also known as the transferrin receptor, is a glycoprotein highly expressed on the surface of proliferating cells. It plays a crucial role in mediating cellular uptake of iron through the binding and internalization of transferrin-bound iron, essential for DNA synthesis and cellular proliferation. This makes CD71 a significant biomarker for studying cell growth and differentiation, as well as for cancer and hematological research.
| Product Name: | Anti-Human CD71 In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0017 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | T56/14 |
| Protein: | CD71 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Transferrin Receptor |
| Isotype: | Mouse IgG1 |
| Reactivity: | Human |
| Applications: | FC, IHC |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | FC, IHC |
| Recommended Usage: | FC WB |
| Reactivity: | Human |
| Host Species: | Mouse |
| Specificity: | Clone T56/14 recognizes human CD71. |
| Antigen Distribution: | CD71 is expressed on most proliferating cells, activated lymphocytes, monocytes, macrophages, erythroid progenitors, and brain endothelium. |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD71 is a 95 kD type II homodimeric transmembrane glycoprotein. The function of CD71 is believed to be primarily nutritional. It plays a role in the control of cellular proliferation through facilitation of iron uptake by way of ferrotransferrin binding and the recycling of apotransferrin to the cell surface. Additionally, transferrin receptor is required for erythropoiesis and proper neurological development, and it has also been suggested that a growth signal might be generated by the transferrin/transferrin receptor interaction. Current genetic analysis indicates that the structural genes for transferrin receptor and for a melanoma-associated antigen (p97), and perhaps transferrin itself, each map to a common chromosome in humans. These proteins exhibit primary sequence homology with transferrin and have the ability to bind ferric iron. Therefore, it is thought that genetic rearrangements in this iron transport region may be associated with malignant transformation. Hence, anti-CD71 mAbs are thought to have therapeutic potential in cases of human leukemia & lymphoma.
| Technical Datasheet: | View |
| Protein: | CD71 |
| Ligand/Receptor: | Transferrin |
| Research Area: | Immunology |
| Mouse IgG1 Isotype Control [HKSP84] | |
|---|---|
| Clone | HKSP84 |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Ultra Low Endotoxin |
| Mouse IgG1 Isotype Control [HKSP] | |
|---|---|
| Clone | HKSP |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Ultra Low Endotoxin |
