The Mesothelin Monoclonal Antibody (CAB9180) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, shows high reactivity with mesothelin in human samples and is validated for use in various applications, including Western blotting.Mesothelin, a biomarker overexpressed in various cancers such as mesothelioma, ovarian cancer, and pancreatic cancer, has been identified as a promising target for cancer diagnosis and therapy.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Mesothelin Monoclonal Antibody
SKU:
CAB9180
Size:
20μL, 100μL
Reactivity:
Human, Rat
Clone Number:
ARC1464
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Purification Method
Affinity purification
Gene ID
10232
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Mesothelin Rabbit mAb (CAB9180) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Confocal imaging of paraffin-embedded Rat lung using Mesothelin Rabbit mAb (CAB9180,dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007,dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.
