The MonoMethyl-Histone H4-K20 Polyclonal Antibody (CAB20823) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, is highly specific and reactive with monomethylated histone H4 at lysine 20 in human samples. It has been validated for use in Western blot and immunoprecipitation applications, allowing for accurate detection and analysis of this important histone modification.Histone H4 lysine 20 monomethylation is known to play a crucial role in regulating gene expression and chromatin structure, with implications for various cellular processes including DNA repair, replication, and transcription.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
MonoMethyl-Histone H4-K20 Polyclonal Antibody
SKU:
CAB20823
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
RHRK V
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Purification Method
Affinity purification
Gene ID
8359
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MonoMethyl-Histone H4-K20 Rabbit pAb (CAB20823) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
