Anti-Mouse CD183 (Clone CXCR3-173) In Vivo Antibody

Product Name:	Anti-Mouse CD183 (Clone CXCR3-173) In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0269
Size:	1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:	CXCR3-173
Protein:	CXCR3
Product Type:	Monoclonal Antibody
Synonyms:	CXCR3G, protein-coupled receptor 9 (GPR9), CKR-L2, IP10 receptor (IP10-R), Mig receptor (Mig-R )
Isotype:	IgG
Reactivity:	Mouse
Applications:	FC, In Vivo, N
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Product Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Applications:	FC, In Vivo, N
Reactivity:	Mouse

Host Species:	Armenian Hamster
Specificity:	CXCR3-173 activity is directed against murine CD183 (CXCR3).
Antigen Distribution:	CXCL3 is expressed on primary memory phenotype CD4+ and CD8+ T cells, naturally occurring CD4+CD25+ Foxp3+ regulatory T cells, natural killer (NK) T cells, and approximately 25% of NK cells. CXCR3-173 recognizes an epitope of CXCR3 expressed on the surface of activated mouse splenocytes.
Immunogen:	Mouse N-terminus of CXCR3
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:	≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.

CXCR3 (CD183) is a chemokine receptor that binds to three ligands, CXCL9 (MIG), CXCL10 (IP-10), and CXCL11 (ITAC), that are induced by IFNgamma, -α/β, or other proinflammatory cytokines1. CXCR3 is important for natural killer (NK) cell-dependent priming of CD4+ T cells in lymph nodes2, host responses to infection3, and CD4+ T cell responses to allografts4,5.

CXCR3-173 was generated by immunizing Armenian hamsters with a peptide sequence unique to mouse CXCR3 which encompasses amino acids 1-376,7. Hamsters were tested by ELISA for seropositivity against CXCR3 peptide and hybridomas were generated, screened by FACS, purified, and tested for: staining, CXCR3 blockade in vitro, and endotoxin levels6. CXCR3-173 detects the native form of CXCR3, and therefore does not work in Western blotting.

CXCR3-173 has potential use in immunotherapeutic approaches to inhibit transplant rejection and immune related diseases6. In vitro, CXCR3-173 blocks chemotaxis in response to CXCL10 or CXCL11 but not CXCL9. In vivo, CXCR3-173 prolongs both cardiac and islet allograft survival in a manner further enhanced by rapamycin.

Protein:	CXCR3
Research Area:	Immunology
Shipping:	2-8°C
NCBI Gene Bank ID:	12766
UniProt:	CD183