![Anti-Mouse CD54 [YN1/1.7.4] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/214397/569475/anti-mouse-cd54-yn11.7.4-in-vivo-antibody-low-endotoxin__21298.1772095077.jpg?c=2?imbypass=on "Anti-Mouse CD54 [YN1/1.7.4] In Vivo Antibody - Low Endotoxin")
Description
Anti-Mouse CD54 [YN1/1.7.4] In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CD54 [YN1/1.7.4] In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD54 protein, also known as ICAM-1 (Intercellular Adhesion Molecule 1), which plays a critical role in the immune response by facilitating leukocyte endothelial transmigration, making it ideal for research in immunology and related fields. With a rat IgG2b kappa isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), perfect for ELISA, flow cytometry, immunohistochemistry, and other assays.
Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy. Enhance your research with this reliable and versatile antibody. CD54 is a glycoprotein expressed on the surface of various cell types, including endothelial cells and immune cells like T-cells, B-cells, and macrophages. It plays a significant role in the immune system by mediating adhesion and signaling processes that are essential for effective immune responses.
| Product Name: | Anti-Mouse CD54 [YN1/1.7.4] In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0062 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | YN1/1.7.4 |
| Protein: | CD54 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Ly-47, ICAM-1 |
| Isotype: | Rat IgG2b κ |
| Reactivity: | Mouse |
| Immunogen: | Mouse NS-1 cells |
| Applications: | CyTOF®, FA, FC, IHC FF, IP, PhenoCycler®, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | CODEX®, CyTOF®, FA, FC, IHC FF, IP, WB |
| Recommended Usage: | FC The suggested concentration for this YN1/1.7.4 antibody for staining cells in flow cytometry is ≤ 0.25 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this YN1/1.7.4 antibody for use in western blotting is 1-10 µg/ml. |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone YN1/1.7.4 recognizes an epitope on mouse CD54. |
| Antigen Distribution: | CD54 is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes. |
| Immunogen: | Mouse NS-1 cells |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
ICAM-1 is a 55 kDa glycoprotein that is part of the Ig superfamily. It is heavily glycosylated to form 75 kDa to 115 kDa. ICAM-1 is known to be an adhesion and viral entry molecule, and its long suspected involevement in signal transduction is being elucidated. The signal-transducing functions of ICAM-1 appear to be mainly associated with proinflammatory pathways. Furthermore, ICAM-1 signaling appears to act as a beacon for inflammatory immune cells such as macrophages and granulocytes bringing about inflammation via lymphocyte trafficking. ICAM-1 is essential for the transmigration of leukocytes out of blood vessels and into tissues, and is a marker of endothelial dysfunction leading to damaging vascular disorders in umbilical and placental vascular tissue of gestational pregnancies. ICAM-1 is the receptor for rhinoviruses (the cause of most common colds) and malaria, and plays an inflammatory role in ocular allergies.
| Technical Datasheet: | View |
| Protein: | CD54 |
| Function: | Immune reaction, inflammation, adhesion |
| Ligand/Receptor: | CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen |
| Research Area: | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cell |
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