Anti-Mouse/Human IL-5 In Vivo Antibody

Product Name:	Anti-Mouse/Human IL-5 In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0364
Size:	1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:	TRFK5
Protein:	IL-5
Product Type:	Monoclonal Antibody
Synonyms:	BCDFII,Interleukin-5, IL5, B cell differentiation factor 1, TRF, EDF
Isotype:	IgG1
Reactivity:	Mouse
Applications:	CyTOF®, Depletion, ELISA Cap, ELISPOT, FA, FC, IHC FFPE, In Vivo, N, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Product Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Applications:	CyTOF®, Depletion, ELISA Cap, ELISPOT, FA, FC, IHC FFPE, In Vivo, N, WB
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Clone TRFK5 recognizes mouse/human IL-5.
Antigen Distribution:	Mast cells, T cells, eosinophils
Immunogen:	Partially-purified T cell clone supernatant
Concentration:	≥ 2.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:	≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	This Low Endotoxin Functional Formulation, GOLD Antibody is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

IL-5 is a 26 kD TH2 cytokine and a homodimeric glycoprotein that is part of the hematopoietic family. IL-5 stimulates B cell growth as well as increases Ig secretion. It is the main factor that promotes the terminal differentiation of eosinophil progenitors and enhances the effector capacity of mature eosinophils. IL-5 is a major regulator of eosinophil accumulation in tissues. In fact, in Hodgkin lymphoma, the characteristically observed eosinophilia is suspected to result from an increased production of IL-5. IL-5 may also be the cause of several allergic diseases including asthma, where it is thought that eosinophils have a significant role in the disease pathology. There are several mAbs currently in use that target IL-5 for the treatment of severe eosinophilic asthma.

Protein:	IL-5
Function:	Growth/differentiation of B cells, eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
Shipping:	2-8°C
PubMed:	IL-5
NCBI Gene Bank ID:	161913567
UniProt:	IL-5