Description
Anti-Mouse Ly-6G/Ly-6C (Gr-1) In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse Ly-6G/Ly-6C (Gr-1) In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the Ly-6G/Ly-6C (Gr-1) antigens, which are critical markers for granulocytes and monocytes, making it ideal for research in immunology and related fields. With a rat IgG2b isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), perfect for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy.
Enhance your research with this reliable and versatile antibody. The Ly-6G/Ly-6C (Gr-1) antigens are commonly found on the surface of granulocytes and a subset of monocytes in mice. These markers play a significant role in the immune system, particularly in the differentiation and identification of neutrophils and monocytes, contributing to the understanding of the innate immune response and inflammation processes.
| Product Name: | Anti-Mouse Ly-6G/Ly-6C (Gr-1) In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0091 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | RB6-8C5 |
| Protein: | Ly-6G/Ly-6C |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Gr-1 |
| Isotype: | Rat IgG2b |
| Reactivity: | Mouse |
| Immunogen: | Raised against granulocytes of mouse origin |
| Applications: | CyTOF®, Depletion, IHC FF, IP, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | CyTOF®, Depletion, IHC FF, IP, WB |
| Recommended Usage: | FC The suggested concentration for this RB6-8C5 antibody for staining cells in flow cytometry is ≤ 0.06 µg per 106 in a volume of 100 µl or 100 µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Rat Anti-Mouse GR-1, as known as anti-mouse Ly-6G/Ly-6C (Clone RB6-8C5) has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| Antigen Distribution: | The Gr-1 antigen is present at various levels correlated with granulocyte differentiation and maturation. The Gr-1 antigen is expressed on other myeloid populations, but not on lymphoid or erythroid cells.1,2 |
| Immunogen: | Raised against granulocytes of mouse origin |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
Gr-1 is a 21-25 kD protein. This myeloid differentiation antigen is a glycosylphosphatidylinositol-linked protein expressed on granulocytes and macrophages. Clone RB6-8C5 antibody has been shown to inhibit the binding of the clone 1A8 antibody. Clone 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C.
| Technical Datasheet: | View |
| Protein: | Ly-6G/Ly-6C |
| Research Area: | Immunology, Innate Immunity |
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