Anti-Mouse TCR V gamma 2 In Vivo Antibody

Product Name:	Anti-Mouse TCR V gamma 2 In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0074
Size:	1mg, 5mg, 25mg, 100mg
Clone:	UC3-10A6
Protein:	V-gamma 2 T-Cell Receptor
Product Type:	Monoclonal Antibody
Synonyms:	V-gamma2 T-cell receptor, T cell receptor gamma 2
Isotype:	Armenian Hamster IgG
Reactivity:	Mouse
Applications:	Depletion, FC, IP
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Applications:	Depletion, FC, IP
Recommended Usage:	FC It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TCR. A suggested method would be to stain cells expressing TCR with Anti-Mouse TCR at ≤1.0 µg per 1.0 X 10⁶ in a 100 µl total staining volume, followed by Goat-anti-Hamster IgG. Each lot of this antibody is quality control tested.
Reactivity:	Mouse

Host Species:	Armenian Hamster
Specificity:	This antibody reacts with V-gamma 2 T-Cell Receptor (TCR)-bearing T lymphocytes.
Antigen Distribution:	V-gamma 2 T-Cell Receptor (TCR)-bearing T lymphocytes make up a significant proportion of γδ TCR-bearing cells in the late fetal and adult thymus and adult peripheral lymphoid tissues and lung.
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an α and β chain in 95% of T cells, while 5% of T cells have TCRs consisting of γ and δ chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules.

Technical Datasheet:	View
Protein:	V-gamma 2 T-Cell Receptor
Function:	Studies suggest that these cells recognize bacterial ligands and some tumor cells.
Ligand/Receptor:	Antigens
Research Area:	Adaptive Immunity, Immunology

Armenian Hamster IgG Isotype Control
Clone	PIP
Isotype	Armenian Hamster IgG
Endotoxin Level	Low Endotoxin

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