null

Anti-PAK1/PAK2/PAK3 Antibody (CAB21090)

SKU:
CAB21090
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
$167 - $395
Frequently bought together:

Description

Antibody Name:Anti-PAK1/PAK2/PAK3 Antibody (CAB21090)
Antibody SKU:CAB21090
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PAK1/PAK2/PAK3 (NP_002567.3).
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, HeLa, MCF7, Mouse testis, Rat testis, C6
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PAK1/PAK2/PAK3 (NP_002567.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GSLT DVVT ETCM DEGQ IAAV CREC LQAL EFLH SNQV IHRD IKSD NILL GMDG SVKL TDFG FCAQ ITPE QSKR STMV GTPY WMAP EVVT RKAY GPKV DIWS L
Cellular Location:
Calculated MW:65kDa
Observed MW:61KDa, 66-68KDa
Synonyms:PAKalpha
Background:This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Apr 2010
Western blot analysis of extracts of various cell lines, using PAK1/PAK2/PAK3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Western blot analysis of extracts of various cell lines, using PAK1/PAK2/PAK3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Related Products