The Phospho-CARM1-S228 Antibody (CABP0319) is a high-quality antibody developed for reliable detection and analysis of target proteins. CARM1, also known as coactivator-associated arginine methyltransferase 1, is a key regulator of gene expression and plays a crucial role in various cellular processes including transcriptional regulation, cell cycle progression, and tumorigenesis.This polyclonal antibody, generated in rabbits, is highly specific to the phosphorylated form of CARM1 at serine 228 and has been validated for use in Western blot applications. By targeting this specific phosphorylation site, researchers can study the activation and function of CARM1 in different cell types and contexts, particularly in the fields of cancer research, epigenetics, and gene regulation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-CARM1-S228 Antibody
SKU:
CABP0319
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
VKSN N
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PRMT4, Phospho-PRMT4/CARM1-S228
Positive Sample:
Jurkat treated with Calyculin A
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
66kDa
Observed MW:
63kDa
This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9.
Purification Method
Affinity purification
Gene ID
10498
RRID
AB_2770958
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Phospho-PRMT4/CAAbGn1-S228 Rabbit pAb (CABP0319) at 1:1000 dilution. Jurkat cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
