The Phospho-CARM1-S228 Antibody (CABP0319) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9. RRID AB_2770958 Gene ID 10498 Swiss Prot Synonym PRMT4; Phospho-PRMT4/CARM1-S228
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-CARM1-S228 Antibody
SKU:
CABP0319
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PRMT4, Phospho-PRMT4/CARM1-S228
Positive Sample:
Jurkat treated with Calyculin A
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
66kDa
Observed MW:
63kDa
This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9. RRID AB_2770958 Gene ID 10498 Swiss Prot Synonym PRMT4; Phospho-PRMT4/CARM1-S228
Purification Method:
Affinity purification
Gene ID:
10498
RRID:
AB_2770958
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Phospho-PRMT4/CAAbGn1-S228 Rabbit pAb (CABP0319) at 1:1000 dilution. Jurkat cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
